(1970) Nicking activity of an endonuclease. The range of measurement is 10250ng/ml for Hoechst, 25pg/ml1g/ml for PicoGreen, and the dyes are sensitive to GC content. QIAGEN silica gel membrane technology also avoids the handling inconveniences of loose silica resins or slurries and the problem of silica carryover which can interfere with downstream applications. Each technique is described below and includes information on necessary accessories (e.g., equipment). QIAGEN Anion-Exchange Resin 0000107765 00000 n QIAGEN silica gel membrane technology yields high-purity nucleic acids suitable for most molecular biology and clinical research applications, such as restriction digestion, ligation, labeling, amplification, and radioactive and fluorescent sequencing. The cells or tissues are digested with Proteinase K in the presence of EDTA to inhibit DNases. 0000021317 00000 n Here at Promega, your success is important to us and we genuinely enjoy the challenge of identifying the right product to address your technical needs. The purified DNA extracted using the PureFood Kit is ready to be used for several applications, including real-time PCR, gel electrophoresis, next-generation and Sanger sequencing and microarrays. The same samples of DNA isolated by five different purification methods in the fragment analyzer trace and DV200 table above were quantitated by qPCR assays of various targets and fragment sizes. This is true even for DNA pellets. Our understanding of genetic material has increased substantially since Friederich Miescher performed the first DNA extraction in 1869. Physical methods are often used with more structured input materials, such as tissues or plants. Spin columns enhance the process of nucleic acid purification making it a lot faster. Up to 95% recovery is achieved, depending upon the DNA fragment size (see Table 7). To protect your privacy, your account will be locked after 6 failed attempts. Expression of the bacterial APH (aminoglycoside phosphotransferase) gene (derived from Tn5). The purified 0000002470 00000 n Figure 2. Nucleic acids prepared on QIAGEN resin are of equivalent or superior purity to nucleic acids prepared by two rounds of purification on CsCl gradients. To protect your privacy, your account has been locked after 6 failed login attempts. After binding DNA, an external magnetic field attracts the beads to the outer edge of the containing tube, immobilizing them. This guide provides a comprehensive introduction to DNA and RNA purification methods, including the basics of DNA isolation, plasmid growth and nucleic acid quantification. Specialized, sample-type specific purification kits may be needed for more complex and challenging samples that contain degraded DNA or a have low concentrations of DNA. This can help you assess not only the integrity of the nucleic acids, but also the likelihood of an amplification-based assay to be successful. Incubate this secondary culture for 1216 hours before harvesting cells. With the target material bound, the flow-through can be removed. Amplifiable genomic DNA can be isolated from up to 5 106 cells, 20mg of tissue or up to 1.2cm of a mouse tail tip without centrifugation of the lysate prior to purification. Xin Q, Cheng J, Wang H, Zhang W, Lu H, Zhou J, Lo GV, Dou Y, Yuan S. RSC Adv. Optimized automated methods are preloaded, the prefilled reagent cartridges are snapped into place, your sample is added and you select "Start" to begin the appropriate method. Products using QIAGEN anion-exchange technology. Percent Recovery Versus Starting DNA Using the ReliaPrepDNA Clean-Up and Concentration System. Figure 13. For high quality, purified cell-free DNA from plasma samples, we offer the Maxwell RSC ccfDNA Plasma Kit (Cat.# AS1480). Silica based salting out offers better resolution and easier recovery of proteins, DNA and other macromolecules. use in most downstream 0000001748 00000 n The Wizard Magnetic 96 DNA Plant System has been validated with corn and tomato leaf as well as with canola and sunflower seeds. Elution points of different nucleic acids from QIAGEN Resin as a function of pH and NaCl concentration. For plasmid miniprep purification, the MagneSil PMPs are used for both lysate clearing and DNA binding, eliminating the need for centrifugation or vacuum filtration, as the binding of nucleic acids occurs in solution. optimal results in sensitive Lis, J.T. A chaotrope denatures biomolecules by disrupting the shell of hydration around them. We investigate the DNA-silica binding mechanism using molecular dynamics simulations. The Chelex method of DNA extraction is suitable for extracting the DNA from a smaller amount of samples. Researchers have used this simple and rapid system for many additional sample types and applications including mosquitoes (16), mammary stem cells (17), Bacillus subtilis (18), Escherichia coli (19), the larval form of the Schistosoma mansoni parasite (20) and viral DNA from Kaposis sarcoma herpes virus-infected BC3 cells (21). The key advantage of QIAGEN anion-exchange resin arises from its exceptionally high charge density. Beyond this time, the separation characteristics of the resin will begin to change, and it will no longer be effective. To increase the yield from the Wizard Magnetic 96 DNA Plant System, a scale up in volume with up to 5 leaf punches can be used [as demonstrated in Promega Notes 79]. An automated method for the Wizard MagneSil Tfx System has been developed for the Biomek FX robotic workstation. For example, its often the case that PCR products can be used directly in T-vector cloning. and Quigley, M. (1981) A rapid boiling method for the preparation of bacterial plasmids. Explore our DNA extraction portfolio to discover the right solution for your purification needs. Remove any extra proteins and other contaminants from the mixture by centrifugation. 0000067201 00000 n Exercise concerning these in next generation sequence (NGS) is a priority. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Silica based salting out has a high tolerance to impurities, which makes it ideal for purifying proteins, DNA and other macromolecules. measurement, a 1:10 dilution is typically used (e.g., 0.1ml culture in 0.9ml culture medium) to keep the reading in the range of 0.11.0, where the spectrophotometer is most accurate. The purified, high-quality DNA is then ready to use in a wide variety of demanding downstream applications, such as multiplex PCR, coupled in vitro transcription/translation systems, transfection and sequencing reactions. As a magnetic particle mover, not a liquid handler, the Maxwell RSC additionally offers several advantages over other automated systems. Figure 20. PCR fragments of 100, 200, 300, 500 and 1,000 base pairs were purified using the Wizard SV 96 PCR Clean-Up System on the Biomek 2000 robotic workstation. 0000003578 00000 n Purified (P) and unpurified (U) fragments were separated on an ethidium bromide-stained, 2% agarose gel. The yield of genomic DNA from the ReliaPrep Blood gDNA Miniprep System varies with white blood cell count. SDS removal steps are incorporated into the QIAGEN protocols. FFPE samples can have a wide-ranging yield of DNA or RNA often as little as 10ng or less in a volume ranging from 10l to 100l from an extraction. The Vac-Man 96 Vacuum Manifold. All lanes contained 10l of reaction product separated on a 1% agarose gel. This can result in sample concentrations below the NanoDrops linear range. Buffers, such as MOPS, sodium phosphate, TrisCl and sodium acetate can be used at the indicated pH. The kit effectively eliminates laborious sample preprocessing steps such as enzymatic pretreatment, as it works with inhibiting sample types and also has the ability to lyse both Gram+ or Gram bacteria. Figure 1. The Wizard MagneSil Tfx System provides a simple and reliable method for the rapid isolation of transfection-quality plasmid DNA in a multi-well format. HiSpeed Midi Tips, provided in the HiSpeed Plasmid Midi Kit, contain a newly developed anion-exchange resin. Results show the mean and standard deviation for 6 purified fragments of each size. Nanomaterials | Free Full-Text | Evolutionary Progress of Silica Consult a centrifuge instruction manual for conversion of rpm to g-force. Eluting and storing the DNA in TE buffer, for example, is helpful as long as the EDTA does not impact your chosen downstream applications. Meanwhile, the buffer also reduces the activity of water by formatting hydrated ions. Promega has performed a thorough investigation of methods at different points in the purification process to ensure the isolation of high-quality DNA from EndA+ (wildtype) bacterial strains. Column-based method to simultaneously extract DNA, RNA, and proteins from the same sample. In terms of sensitivity in nucleic acid detection, it is surpassed only by ddPCR. The DNA purified from many of these samples can be used in PCR-based testing for Genetically Modified Organism (GMO) DNA sequences, such as by quantitative analysis using TaqMan assays. Utilizing spin, vacuum or magnetic-based methods, our manual single-prep solutions are best for processing less than 24 samples at a time. Each of these chemistries can influence the efficiency and purity of the isolation, and each have a characteristic binding capacity. SPE is used to isolate a species in a sample or to clean-up a sample before analysis. Effect of Various PCR Additives on Percent Recovery of a 1,000bp PCR Product Using the Direct Purification Method and the Wizard SV Gel and PCR Clean-Up System. How does temperature affect DNA extraction? - Quick-Advice.com Thus, any further replication is prevented until after the two plasmids have been segregated to different cells to create the correct prereplication copy number (40). Chelex resin also inhibits DNA degradation by chelating metal ions. 0000003523 00000 n In our experience, transfection experiments with HeLa and NIH/3T3 cells demonstrated that there was little DNA preparation difference with four different plasmid isolation systems used (based on silica membrane, anion exchange and silica resin) when comparing efficiencies using the same transfection reagent. Binding to silica is not DNA specific, so if pure DNA is required, there is also the option to add ribonuclease (RNase A) to the elution buffer. Please request another reset link. Singh, U. There was an issue sending the verification email. This system is of technological importance, and also of interest to explore how negatively charged DNA can bind to a silica surface, which is also negatively charged at pH values above its isoelectric point near pH 3. QIAGEN resin is stable for up to six hours after equilibration. (1962) The effect of electrolytes on the stability of the deoxyribonucleate helix. The high concentration of salt causes the proteins to fall out of solution, and then centrifugation separates the soluble nucleic acid from the cell debris and precipitated protein (1). This may be important, as some cultured cells are sensitive to the amount of endotoxin and other contaminants present in the plasmid preparation. Maxwell purification chemistries use novel magnetic particle-based solutions that naturally decrease contamination carryover. However, use of LB-Miller medium containing more NaCl will produce significantly greater yields and is highly recommended. The process takes longer than the Chelex 100 and involves more than one change of tube and so increases the possibility of sample mixing and cross-contamination. The extraction of DNA from semen and very small bloodstains using . DNA is soluble in low-ionic-strength solution such as TE buffer or nuclease-free water. Maxwell HT Systems allow purification of DNA or RNA at scale on any laboratory liquid handler in 24- or 96-well SLAS format. eCollection 2022 Feb 22. 0000002448 00000 n Chelex resin (Chelex 100) is a specialized resin that chelates metal ions as well as other contaminants (Chelex = Chelating Ion Exchange Resin). This method relies on the fact that nucleic acid will bind to the solid phase of silica under certain conditions. Stay notified of Promega events, products and news. Shi, R. L. (2018). This leads to the silica surface and DNA becoming dehydrated. Paithankar, K.R. Blood sample was thawed, allowing for DNase activity. Polysaccharides and proteins do not bind well to the column and residual traces are removed during alcohol-based wash steps, along with the salts. It is based on the principle of binding nucleic acid to immobilized solid-phased spin columns of different materials under specific circumstances. Spin column-based nucleic acid purification. Wolfe, et al. The five-step, ~100 minute protocol requires only 30 minutes of hands-on time, effectively achieving not only faster results with walk-away automation, but also freeing up laboratory resources for higher value activities. The sample in binding solution is then transferred to a spin column, and the column is put either in a centrifuge or attached to a vacuum. Fragment DNA purification can improve efficiency in subsequent reactions. Promega products like the Wizard Plus SV Minipreps DNA Purification System (Cat.# A1330, A1460, A1465) and the PureYield Plasmid Systems combine the benefits of alkaline lysis with the rapid and easy purification by silica. While there are general trends, the DV200 score does not necessarily correlate with success in downstream assays such as qPCR. This in-turn provides you with high-quality material for different experiments like cloning and long-range sequencing. Natural Treatments to get rid of Lower Back Pain, Anxiety and Panic Attacks Holistic Treatments, Human Anatomy and Physiology Study Course, Within molecular biology generally, the 'salting out' procedure has been widely used. By avoiding the need for centrifugation or vacuum filtration, DNA purification with the Wizard MagneSil Tfx System can be completely automated, requiring the MagnaBot 96 Magnetic Separation Device and Heat Transfer Blockfor the protocol. Promega was one of the first companies to provide kits for the purification of DNA, as well as plasmids, with over 30 years of experience in nucleic acid extraction. Unable to load your collection due to an error, Unable to load your delegates due to an error. (Vac-Man 96 Vacuum Manifold, Cat.# A2291) and a vacuum pump capable of generating 1520 inches of mercury or the equivalent. Before Figure 5. Bacterial growth in liquid culture occurs in three phases: 1) a short lag phase in which the bacteria become acclimated to the media and begin to divide; 2) a log phase, characterized by exponential growth in which most strains of E. coli will divide every 2030 minutes; and 3) a stationary phase in which growth slows and eventually stops in response to the lack of nutrients in the medium. The ReliaPrep Blood gDNA Miniprep System processes 200l of blood or body fluid, either fresh or frozen, in less than 40 minutes. When harvesting bacteria, follow the conditions outlined in either the Wizard Plus SV Miniprep DNA Purification System or the PureYield Plasmid Midiprep Systemprotocol. The soluble plasmid DNA is ready to be further purified. Akash Gautam . The goal of lysis is to rapidly and completely disrupt cells in a sample to release nucleic acid into the lysate. Most laboratories have a NanoDrop Microvolume Spectrophotometer (or similar device) and they are incredibly easy to use. The salt concentration and pH conditions of the buffers used determine whether DNA is bound or eluted from the column. https://doi.org/10.1016/0923-2508(92)90107-y, CrossRef The chemical formula of EDTA is C 10 H 16 N 2 O 8. (1975) The differential precipitation of nucleic acids and proteins from aqueous solutions by ethanol. In contrast, conventional anion-exchangers, based on cellulose, dextran, or agarose, have separation ranges only up to 0.4 M salt, so that binding and elution of all substances is limited to a narrow range of salt concentrations. A common method of physical disruption is freezing and grinding samples with a mortar and pestle under liquid nitrogen to provide a powdered material that is then exposed to chemical or enzymatic lysis conditions. Once extracted, the resulting DNA is ready for advanced downstream molecular analyses, including serotyping, NGS and identification of spoilage organisms. Pipette 1-2l of sample, select Analyze and the instrument provides a read out of concentration and purity via A260/A280 and A260/A230 ratios in just a few seconds. Troubleshooting Guide for Genomic DNA Extraction & Purification - NEB Copy number is determined primarily by the region of DNA surrounding and including the origin of replication in the plasmid. qPCR can also provide a measure of how degraded or crosslinked a DNA sample may be since nucleic acid must be a suitable substrate for a DNA polymerase for a signal to be generated. J Clin Microbiol. Figure 19. The pGL4.13[luc2/SV40] Vector (Cat.# E6681) was prepared using a competing system or the PureYield Plasmid Miniprep System. Silica aerogels have played a dominant role in both academics and industry since their first report in the 1930s . There are two main considerations when using a NanoDrop: sensitivity and integrity. Here's what happens during the process: 1. Anal Methods. Solid Phase Extraction (SPE) Solid phase extraction1 (SPE) is a sample preparation technique using a solid adsorbent contained most commonly in a cartridge device (Figure 1), or on a disk to adsorb select species from solution. Nucleic acid binds to cellulose in the presence of high salt and alcohols. Singer-Sam, J., Tanguay, R. L., & Rjggs, A. O. Plate Readers, Fluorometers & Luminometers, Small Molecule Profiling and Assay Development, Wizard Plus SV Miniprep DNA Purification System, Wizard Plus SV Plasmid DNA Purification System Technical Bulletin, Factors that Affect Plasmid DNA Quality and Yield, DNA Fragment Purification from Agarose Gels and PCR Amplification, Methods for Determining DNA Yield & Purity by Absorbance. A reading at 320nm will indicate if there is turbidity in the solution, another indication of possible contamination. Your purified DNA is ready for analysis in about 50 minutes, and can be used directly in various downstream applications, such as agarose gel electrophoresis. When considering FFPE samples, it is important to note that dye-based quantitation does not estimate the integrity of the DNA/RNA or the extent of cross-linking in the sample, which could affect success in downstream assays. The percentage of agarose in the gel will determine what size range of DNA will be resolved with the greatest clarity (40). A vacuum manifold or a microcentrifuge is used for sample processing. The names of the different QIAGEN-tips indicate the binding capacities (in g) of the columns for double-stranded plasmid DNA, as determined with purified pUC18 DNA. DNA separation by silica adsorption is a method of DNA separation that is based on DNA molecules binding to silica surfaces in the presence of certain salts and under certain pH conditions.[1][2]. Incubation with shaking for 816 hours at 37C before harvesting generally results in maximum yields of a high-copy-number plasmid. Like the other cookies we use, strictly necessary cookies may be either first-party cookies or third - party cookies. Chang, C. N. (2008). Our records indicate that this email address is already registered. Frontiers in Genetics, 11, 374. DNA separation by silica adsorption - Wikipedia We use cookies and similar technologies to make our website work, run analytics, improve our website, and show you personalized content and advertising. Panel B. DNA yields as determined using the QuantiFluor dsDNA System. Thawing frozen blood samples releases DNase, causing degradation. The DNA can then be washed with high salt and ethanol, and ultimately eluted with low salt. A bacteriostatic agent that interferes with bacterial protein synthesis by binding to the 50S subunit of ribosomes and preventing peptide bond formation. A., Kumari, M., & Iyengar, S. (2018). Spin column-based nucleic acid purification - Wikipedia and transmitted securely. For small PCR fragments (<500bp), optimal recovery requires a 95% ethanol wash. For larger fragments (>500bp), optimal results are achieved using an 80% ethanol wash. The low elution volume is possible because the column design retains virtually no buffer. Our team of automation experts offer assistance to help develop and implement an automated nucleic acid purification solution customized to the needs of your laboratory. Simplifying Nucleic Acid Extraction and Cutting Down Waste Once extracted, DNA can be used for molecular analyses including PCR, electrophoresis, sequencing, fingerprinting and cloning. (2009). After an overnight Proteinase K digestion, genomic DNA can be manually purified from FFPE thin tissue sections in less than an hour. The remaining tissue is discarded. Concentration (Panel A), total yield (Panel B) and purity (Panel C) were assessed using absorbance spectroscopy. One microliter of purified genomic DNA was amplified using PCR Master Mix (Cat.# M7502) and mouse-specific IL-1 primers (1.2kb product). Molecular diagnostic applications in forensic science. Yields from blood are typically 410g, depending on the white blood cell count. silica-membranes, silica-covered magnetic beads, or anion-exchange columns), which specifically bind DNA and subsequently release it to . Depending on inoculation size and the size of the culture, stationary phase will be reached in 68 hours. divided by 1.5 O.D./ml = 2.67ml). Magnetic DNA Purification: History and recent developments - Sepmag In: DNA and RNA Isolation Techniques for Non-Experts. https://doi.org/10.1007/978-3-030-94230-4_5, DOI: https://doi.org/10.1007/978-3-030-94230-4_5, eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0). 0000021851 00000 n For larger cultures with volumes ranging from 50100ml, the PureYield Plasmid Midiprep System (Cat.# A2492, A2495, A2496) is a good choice. 0000024247 00000 n Huh-7 cells were transfected using 200 ng plasmid DNA and 0.5 l Attractene Transfection Reagent or 300 ng plasmid DNA and 0.75 l Attractene Transfection Reagent. It is a colorless (white as in powder form), water-soluble and organic molecule. Agarose gel electrophoresis of the purified DNA eliminates some of the issues associated with absorbance readings. The same samples of DNA isolated by five different purification methods in the fragment analyzer trace and DV200 table above were quantitated by qPCR assays of various targets and fragment sizes. Forensic Science International: Genetics, 44, 102191. Rapid and simple method for purification of nucleic acids. CrossRef Spin columns contain a silica resin that selectively binds DNA, depending on the salt conditions and other factors influenced by the extraction method. RNA acts as a competitive inhibitor and alters the endonuclease specificity from that of a double-stranded nucleolytic enzyme yielding seven-base oligonucleotides to a nickase that cleaves an average of one time per substrate (3536). The A600 of a tenfold dilution of the culture should be 0.100.35. DNA is more stable at a slightly basic pH and will dissolve faster in a buffer. BioMed Research International, 2009, 574398. Method for quickly extracting and preserving silica gel membrane type DNA Fragment analyzer trace of DNA isolated from FFPE sections using five different purification methods. BioTechniques, 54(3). The Maxwell RSC Buffy Coat DNA Kit (Cat.# AS1540) provides a simple, automated method of genomic DNA extraction using the convenient, prefilled cartridge format of the Maxwell RSC Instrument. Another specialized genomic DNA isolation system is the Wizard Magnetic DNA Purification System for Food (Cat.# FF3750, FF3751). 0000003901 00000 n is the measure of how much light is blocked by the biomass of the bacterial culture in a path length of 1cm. 0000002017 00000 n 2023 Feb 16;15(7):916-924. doi: 10.1039/d2ay01549h. Suspend the pellet in a buffer containing a chaotrope, this will cause the DNA to be released from the silica. A one-step microbial DNA extraction method using Chelex 100 suitable for gene amplification. Solid-phase DNA extraction relies on the binding of DNA to a silica support in the presence of a chaotropic salt at pH 7.5; this is below the pKa of the surface silanol groups and so reduces the negative charge at the surface thereby decreasing electrostatic repulsion and facilitating DNA adsorption [2]. PubMedGoogle Scholar. Lane M, 1kb DNA Ladder (Cat.# G5711). BioTechniques, 43(6), 799804. The lysis buffer destabilizes the cell membranes, leading to the breakdown of cellular structure. While all methods are useful, each has caveats to consider when choosing a quantitation approach. A single plate can be processed in 60 minutes or less. Information on genetic markers in bacterial strains can also be found in Ausubel et al. A swinging-bucket tabletop centrifuge or the Eluator Vacuum Elution Device (Cat.# A1071) is required for the final elution step regardless of the protocol chosen. 1989 (39). These kits are generally much easier and faster to use than traditional methods, and do not require significant expertise. DNA prepared using QIAGEN-tips has been tested with restriction endonucleases, polymerases (including Taq DNA polymerase), DNA ligases, phosphatases, and kinases. Although techniques like Southern blotting, which require microgram amounts of DNA, are still performed in molecular biology laboratories, most assessments of chromosomal DNA is done by PCR-based technologies. Purification of nucleic acids with silica gel membrane products is fast, convenient, and economical. (1978) Plasmid-determined resistance to antimicrobial agents. The benchtop-compact Maxwell Instruments are easy to set up and require no special training for use. The endA gene encodes a 12kDa periplasmic protein called endonuclease I. In the past, the process of extraction and purification of nucleic acids used to be complicated, time-consuming, labor-intensive, and limited in terms of overall throughput. The principle behind this type of separation relies on DNA molecules binding to silica surfaces in the presence of certain salts and under certain pH conditions. It essentially combines the classic Buffer 3 of a plasmid prep, which contains acetic acid to neutralize Buffer 2, as well as guanidinium to get that plasmid DNA to bind to the silica. 2015 Aug 11;11(8):3932-45. doi: 10.1021/acs.jctc.5b00286. Development of a 3D-printed single-use separation chamber for use in mRNA-based vaccine production with magnetic microparticles. solid-phase anion-exchange separations Principle QIAGEN resin is a macroporous silica-based resin with a high density of diethylaminoethyl (DEAE) groups that was developed exclusively for isolation of nucleic acids. In addition, DNA can be purified from processed food such as corn chips, chocolate and chocolate-containing foods, lecithin and vegetable oils if used with the appropriate optimized protocols. The resin consists of defined silica beads with a particle size of 100 m, a large pore size, and a hydrophilic surface coating. It is advantageous over other extraction techniques such as less time consumption, economical, energy-efficient, automated operation, prevention from cross-contamination, and high yield. The Wizard SV 96 and SV 9600 Systems are designed for use either in a manual format or with automated instruments. The Plate Clamp 96 (Cat.# V8251) is recommended for automated protocols and is designed to ensure PCR plates are uniformly flat for liquid transfer on a robotic platform. d. magnetic beads coated with silica organic extraction Genes responsible for cell maintenance functions that all cell types need to perform such as replication, transcription, translation and cell division are called a. prostate specific antigens b. ABO blood antigens c. housekeeping genes d. blood biomarkers e. exons housekeeping genes applications We offer two different ReliaPrep gDNA Miniprep Systems that purify genomic DNA using a cellulose column-based method: ReliaPrep Blood gDNA Miniprep System (Cat.# A5081, A5082) and ReliaPrep gDNA Tissue Miniprep System (Cat.# A2051, A2052). Currently one of the most popular RNA extraction kits is the Qiagen RNeasy kit . Hoechst bisbenzimidazole dyes or PicoGreen selectively bind double-stranded DNA (dsDNA). The procedure can be performed in 20 (Midi and Maxi), 40 (Mega), or 50 minutes (Giga) using a vacuum and centrifuge. The Maxwell Instruments are magnetic-particle-handling instruments that efficiently bind nucleic acids to the paramagnetic particle in the first well of a prefilled cartridge. Part of Springer Nature. This 96-well magnet is used for capturing MagneSil PMPs for DNA purification. Separation of soluble and insoluble material is accomplished by a clearing method (e.g., filtration, magnetic clearing or centrifugation). Bind capacity is an indication of how much nucleic acid an isolation chemistry can bind before it reaches the capacity of the system and no longer isolates more of that nucleic acid. The Maxwell HT DNA FFPE Isolation System purifies nucleic acid using paramagnetic particles, which provide a mobile solid phase to optimize binding, washing and purification of gDNA.