Centers for Disease Control and Prevention. xVnF}W-no~ALmI! Comparison of zone sizes to a standardized chart will only provide information on the antibacterials to which a bacterial pathogen is susceptible or resistant. <>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/Annots[ 31 0 R 32 0 R 33 0 R] /MediaBox[ 0 0 612 816.96] /Contents 23 0 R/Group<>/Tabs/S/StructParents 2>>
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 In February 2015, the FDA added new recommendations for careful hand cleaning of the duodenoscope elevator mechanism (the location where microbes are most likely to escape disinfection), and issued more careful documentation about quality control of disinfection protocols (Figure \(\PageIndex{7}\)). The Kirby-Bauer assay starts with a Mueller-Hinton agar plate on which a confluent lawn (bacteria is spread across the entire surface of the plate) is inoculated with a sample or patients isolated bacterial pathogen. Course Hero is not sponsored or endorsed by any college or university. When you see a rainbow the red is at the larger radius with the violet on the inside of the bow. The effectiveness of various chemical disinfectants is reflected in the terms used to describe them. A chemical agent with a phenol coefficient of less than 1.0 is less effective than phenol. 
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 91 0 obj An additional handout, Did you know about detergents?, provides further information about saponification, soap scum and soapless detergents. If you need to go back and make any changes, you can always do so by going to our Privacy Policy page. High-level germicides have the ability to kill vegetative cells, fungi, viruses, and endospores, leading to sterilization, with extended use. <> What is the difference between soaps and detergents? Cleaning is the removal of foreign material (e.g., soil, and organic material) from objects and is normally accomplished using water with detergents or enzymatic products. Thank you for taking the time to confirm your preferences. 
(PDF) Comparing Surfactants: Efficiency and Effectiveness - ResearchGate  For this test, a cylinder of stainless steel is dipped in a culture of the targeted microorganism and then dried. *&p2oyoRo>>m#,)~rx{Wd-`,,xyIZHsEyK/UatF=m*m>.kX9_E;+&+7;Fg  MiW##3Go}Z
 93 0 obj Are plastics the best option for saving energy in our homes, as well as saving the planet? [)Exm	(}gd[Gl9DBT^=ln|yjz>}h>UwMksv]|x8OR>7?K-mfWoaj>+% >7]dg/4[eV"-G\$,+ctwH7Y`1.-Kil
kop({l[L3365>kr5/. <> This website collects cookies to deliver a better user experience. To obtain soap solution from soap flakes - dissolve soap flakes (or shavings from a bar of soap) in ethanol . CDC twenty four seven. II) When soap is mixed . 1 0 obj
 endobj However, unlike the macrobroth and microbroth dilution methods, the MBC cannot be determined with the Etest. To interpret the results, the intersection of the elliptical zone with the gradient on the drug-containing strip indicates the MIC. 4.interference of light. For the best experience on our site, be sure to turn on Javascript in your browser. 0000003977 00000 n   Determining these concentrations helps identify the correct drug for a particular pathogen. Support your answer with the results recorded in *}2ramtz'%g/daY}S_LTzE14u0aTd,23m0Wi>u/F)iOoe-;gH)12ga]oQT}4>n*vwHIvy}O3~!$%En8q}464>Hb,!-O|)XumDtXoJqIn[CMQf_ nKn? 
PDF Cleaning Compound Efficiency - Dtic As a result, physicians rarely have the luxury of conducting susceptibility testing before they write a prescription. In many cases, the prescription is effective; however, in an age of increased antimicrobial resistance, it is becoming increasingly more difficult to select the most appropriate empiric therapy.     (a) A plate is inoculated with various antimicrobial discs. endobj Why is that? Then becoming saturated, which forms a more solid texture. MICs are interpreted as the lowest concentration that inhibits visible growth, the same as for the macrobroth dilution in test tubes. Several CRE outbreaks have been traced to endoscopes, including a case at Ronald Reagan UCLA Medical Center in early 2015 in which 179 patients may have been exposed to a contaminated endoscope. Properties of an ideal disinfectant, Table 3. stream 
data table 2: soap cleaning efficiency tests The Association of Official Agricultural Chemists International (AOAC), a nonprofit group that establishes many protocol standards, has determined that a minimum of 59 of 60 replicates must show no growth in such a test to achieve a passing result, and the results must be repeatable from different batches of disinfectant and when performed on different days. Temperature, pH, and other factors can also affect the potency of a disinfecting agent. Enzymes in these formulations attack proteins that make up a large portion of common soil (e.g., blood, pus). 
PDF Cleaning and sanitization efficiency of some cleansing agents of  1. Cleaning solutions also can contain lipases (enzymes active on fats) and amylases (enzymes active on starches). <> Ultimately, the source of the infection was traced to the duodenoscopes used in the procedures. Ultimately, the end-users (hospitals and their staff) are responsible for following these procedures and can be held liable if a breach occurs and patients become ill from improperly cleaned equipment. Learn everything from how to sign up for free to enterprise use cases, and start using ChatGPT . The extension activity gives students the opportunity to build on their work in Testing shower gels and soaps by planning their own investigation to compare the properties of soap and detergent products. At what point do the risks outweigh the benefits of these devices, and who should be held responsible when negative outcomes occur? trailer The Kirby-Bauer disk diffusion test has long been used as a starting point for determining the susceptibility of specific microbes to various antimicrobial drugs or chemicals.  endobj
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 The MIC is determined by examining the tubes to find the lowest drug concentration that inhibits visible growth; this is observed as turbidity (cloudiness) in the broth. 0000010438 00000 n  hbbd``b`+S` 5, 2 e@ n9	b^	( c $L@z}@30Kg   ~;
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 Please refer to the attachment to answer this question. "x'T+eQFX9}pyN*lq$;mB JGYp8I3%zS11_>72	p,I. Why is that? With manual cleaning, the two essential components are friction and fluidics. corn oil, and coconut oil (samples 1  3), which soap had the best <> 443, 464Another study found no significant difference between enzymatic and non-enzymatic cleaners in terms of microbial cleaning efficacy467. In a November 2014 study published in the journal Infection Control and Hospital Epidemiology, researchers determined that 85% of the prescriptions ordered in skilled nursing facilities were decided upon empirically, but only 35% of those prescriptions were deemed appropriate when compared with the eventual pathogen identification and susceptibility profile obtained from the clinical laboratory. Ten drops, totaling approximately 0.2 mL of this mixture, are then inoculated onto each of two agar plates. You'll get a detailed solution from a subject matter expert that helps you learn core concepts. Several patients who underwent similar procedures at the same hospital also developed CRE infections, some dying as a result. %PDF-1.5
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 Summary of advantages and disadvantages of commonly used sterilization technologies, Table 7. As the bacterial inoculum grows, antibiotic diffuses from the plastic strips into the agar and interacts with the bacterial cells. endobj
 <> %D2Y{:? 5.scattering of light. Summary of advantages and disadvantages of chemical agents used as chemical sterilants or as high-level disinfectants, Table 6. Characteristics of an ideal low-temperature sterilization process, Table 10. Bacterial contamination can be present in used ultrasonic cleaning solutions (and other used detergent solutions) because these solutions generally do not make antibacterial label claims446. Get up and running with ChatGPT with this comprehensive cheat sheet. Reference sources ofsoil and fat mixture to be tested for each type of product. There is no guarantee that new procedures, protocols, or equipment will completely eliminate the risk for infection associated with endoscopes. 457Some data demonstrate that enzymatic cleaners are more effective than neutral detergents465, 466in removing microorganisms from surfaces but two more recent studies found no difference in cleaning efficiency between enzymatic and alkaline-based cleaners. She was also started on aggressive treatment with intravenous administration of a new drug called tigecycline, which has been successful in treating infections caused by drug-resistant bacteria. Thus, cleaning alone effectively reduces the number of microorganisms on contaminated equipment. Explain the significance of the minimal inhibitory concentration and the minimal bactericidal concentration relative to the effectiveness of an antimicrobial drug. Her condition continued to deteriorate, and she developed symptoms of septic shock. 0000006502 00000 n  Who is responsible, however, when negative outcomes occur due to flawed protocols or faulty equipment? to demonstrate the relationship of the fatty acid percents and basic nature of soaps. <> Such products include soaps, detergents, bleaches, waxes, and polishes. 0000001400 00000 n  endobj <> These units sometimes have a cycle that subjects the instruments to a heat process (e.g., 93C for 10 minutes)451. What distance the box will move in 22 sec? <>
 These cookies allow us to count visits and traffic sources so we can measure and improve the performance of our site. Answer: They all had a thick consistency, which ran down smoothly. oIEJJf`Zf;XD[yy|X"AIV.~^*tuz~Qb
"$`\& n30@, stream To treat her kidney infection and septic shock, Roberta was treated with dialysis, intravenous fluids, and medications to maintain blood pressure and prevent blood clotting. Unfortunately, infectious diseases dont take a time-out for lab work. Because multiple strips containing different antimicrobials can be placed on the same plate, the MIC of multiple antimicrobials can be determined concurrently and directly compared. All information these cookies collect is aggregated and therefore anonymous. 
 Some claim that Olympus did not obtain FDA approval for design changes that may have led to contamination, and others claim that the manufacturer knowingly withheld information from hospitals concerning defects in the endoscopes. ew^zf^x%|o(y-9!$862(q8`*Zw{05:+dBj.so`heHH+RmH| -TWvPpVs`( `NcF?i4)1W0Lg, " 2 0 obj
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 Friction (e.g., rubbing/scrubbing the soiled area with a brush) is an old and dependable method. endobj
 Support your answer with the results recorded in Students conduct a practical experiment using samples of 56 shower gels and soaps, determining their pH values and testing their effects on cooking oil. One graph will show the results with water only. Table 2. endobj These soaps and polishes standards help guide product manufacturers in the proper methods of producing and testing such products to ensure quality towards safe use. <>
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*:pFiVymXBp.SxxH,#Ax.TU9g*SR c|65u\#oZ`	"b%P4]P' Unfortunately, protocols are not infallible, and sometimes it takes negative outcomes to reveal their flaws. % Despite the hospital staff meticulously following manufacturer protocols for disinfection, bacteria, including CRE, remained within the instruments and were introduced to patients during procedures. The most common types of mechanical or automatic cleaners are ultrasonic cleaners, washer-decontaminators, washer-disinfectors, and washer-sterilizers. When they have completed their research, students answer a series of questions to help them compare the different products and reflect on how they are used. 104 0 obj endobj
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\ "2 Students apply their knowledge about soaps and detergents to design an experiment investigating the effect of different types of soap on bubbles, with the objective of answering the following questions: This key words handout provides further information about important terms relating to the chemistry of soaps and detergents. 5 0 obj
 endobj Figure 11.8. What is the purpose of adding cleansing agent? (I3?_c$1]D4#]	cD
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 5. What is the governments role in ensuring that use and maintenance of medical equipment and protocols are fail-safe? When comparing the activities of two disinfectants against the same microbe, using the disk-diffusion assay, and assuming both are water soluble and can easily diffuse in the agar, would a more effective disinfectant have a larger zone of inhibition or a smaller one? Stimulate and engage your students to learn about: Each activity includes instructions for students, as well as editable worksheets and resources available for download. 
 Detergents, soaps and surface tension - RSC Education  Surgical instruments should be presoaked or rinsed to prevent drying of blood and to soften or remove blood from the instruments. endobj A box slides down an incline with uniform acceleration.It starts from rest and attains a speed of 18.1 m/s in 6.8 sec. Students choose three shower gels or soap bars to research and analyse. 5 points (upload photos to the end of your lab write -up), Describe saponification. See Data Table 1: Cold Method to see observations, During the second phase, the hot bath phase, the test tubes were placed into a hot bath, This was enough time for the soap to set and the. Solution: 2) I) When soap is mixed with calcium ions, it reacts with calcium and produces scum, which is insoluble in water. Chemical disinfectants are grouped by the power of their activity, with each category reflecting the types of microbes and viruses its component disinfectants are effective against. Course Hero is not sponsored or endorsed by any college or university. endstream Este site coleta cookies para oferecer uma melhor experincia ao usurio. magnesium ions? Historically, the effectiveness of a chemical disinfectant was compared with that of phenol at killing. 0000008847 00000 n  What are the starting reactants and final products? <>
 Why does this happen? Generally, serum levels of an antibacterial should be at least three to five times above the MIC for treatment of an infection. Butter and margarine: whats the difference? creating different soaps this experiment will allow us to physically see changes in. Temperature is a physical property of matter that is vital not only in the soap efficiency, but also in the formation of soap. These standards help identify the certain physical and chemical properties of soaps and polishes such as particle size, pH, cloud point, recoatability, glide adhesion, freeze/thaw resistance, cleaning efficiency, and foaming properties among others. <> 2.transmission of light. Support your answer with the results recorded in Data Table 2. startxref <>
 0000005036 00000 n  The effectiveness of a disinfectant is influenced by several factors, including length of exposure, concentration of disinfectant, temperature, and pH. 10 0 obj
 We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. Hence the water is considered as hard water. As the bacterial inoculum grows, drug or chemical diffuses from the circular disk into the agar and interacts with the growing bacteria. ASTM International is providing no-cost public access to important ASTM standards used in the production and testing of personal protective equipment. As with all chemicals, enzymes must be rinsed from the equipment or adverse reactions (e.g., fever, residual amounts of high-level disinfectants, proteinaceous residue) could result. Of the synthesized soaps produced from canola oil, D501-03(2016) Standard Test Methods of  Sampling and Chemical Analysis of Alkaline Detergents, D500-95(2016) Standard Test Methods of  Chemical Analysis of Sulfonated and Sulfated Oils, D820-93(2016) Standard Test Methods for  Chemical Analysis of Soaps Containing Synthetic Detergents, D502-89(2016) Standard Test Method for  Particle Size of Soaps and Other Detergents, D1768-89(2016) Standard Test Method for  Sodium Alkylbenzene Sulfonate in Synthetic Detergents by Ultraviolet  Absorption, D1570-95(2016) Standard Test Methods for  Sampling and Chemical Analysis of Fatty Alkyl Sulfates, D2022-89(2016) Standard Test Methods of  Sampling and Chemical Analysis of Chlorine-Containing Bleaches, D2357-11(2016) Standard for  Qualitative Classification of Surfactants by Infrared Absorption, D2023-89(2016) Standard Test Method of  Analysis for Sodium Toluene Sulfonate in Detergents, D3598-89(2016) Standard Test Method for  Citrate in Synthetic Detergents, D3048-89(2016) Standard Test Method of  Assay for Alkaline Protease, D4251-89(2016) Standard Test Method for  Active Matter in Anionic Surfactants by Potentiometric Titration, D2358-16 Standard Test Method for  Separation of Active Ingredient from Surfactant and Syndet  Compositions, D3049-89(2016) Standard Test Method for  Synthetic Anionic Ingredient by Cationic Titration, D3673-89(2016) Standard Test Methods for  Chemical Analysis of Alpha Olefin Sulfonates, D2959-95(2017) Standard Test Method for  Ethylene Oxide Content of Polyethoxylated Nonionic Surfactants, D1767-89(2017) Standard Test Method for  Ethylene Diamine Tetraacetate (EDTA) in Soaps or Synthetic  Detergents, D4608-89(2017) Standard Test Method for  Citrate in Detergents, D4337-89(2017) Standard Test Methods for  Analysis of Linear Detergent Alkylates, D4711-89(2017) Standard Test Method for  Sulfonic and Sulfuric Acids in Alkylbenzene Sulfonic Acids, D5547-95(2017) Standard Test Method for  Clay and Zeolite in Powdered Laundry Detergents by Atomic Absorption, D4954-89(2017) Standard Test Method for  Determination of Nitrilotriacetates in Detergents, D5806-95(2017) Standard Test Method for  Disinfectant Quaternary Ammonium Salts by Potentiometric Titration, D4252-89(2017) Standard Test Methods for  Chemical Analysis of Alcohol Ethoxylates and Alkylphenol Ethoxylates, D928-03(2017) Standard Specification for  Sodium Bicarbonate, D2180-17 Standard Test Method for  Active Oxygen in Bleaching Compounds, D1569-05(2014) Standard Test Methods for  Detergent Alkylate (Withdrawn 2023), D1681-05(2014) Standard Test Method for  Synthetic Anionic Active Ingredient in Detergents by Cationic  Titration Procedure (Withdrawn 2023), D460-91(2014) Standard Test Methods for  Sampling and Chemical Analysis of Soaps and Soap Products (Withdrawn 2023), D1568-05(2014) Standard Test Methods for  Sampling and Chemical Analysis of Alkylbenzene Sulfonates (Withdrawn 2023), D6174-01(2006) Standard Test Method for Inorganic Sulfate in Surfactants by Potentiometric Lead Titration (Withdrawn 2015), D800-05(2014) Standard Test Methods of  Chemical Analysis of Industrial Metal Cleaning Compositions (Withdrawn 2023), D6173-97(2014) Standard Test Method for  Determination of Various Anionic Surfactant Actives by Potentiometric  Titration (Withdrawn 2023), D5070-90(2014) Standard Test Method for  Synthetic Quaternary Ammonium Salts in Fabric Softeners by  Potentiometric Titrations (Withdrawn 2023), D3209-93(2015) Standard Test Method for  Freeze/Thaw Resistance of Polymer Floor Polishes, D6827-02(2016) Standard Test Method for  Zinc Analysis of Floor Polishes and Floor Polish Polymers By  Flame Atomic Absorption (A.A.), D5678-17 Standard Test Method for  Freeze/Thaw Resistance of Wax Emulsion Floor Polish, D3716-14(2020) Standard Test Methods for  Use of Emulsion Polymers in Floor Polishes, D4095-97(2020) Standard Practice for  Use of the Refractometer for Determining Nonvolatile Matter  (Total Solids) in Floor Polishes, D3430-95(2021) Standard Test Method for  Clarity and Yellowness of Liquid Water-Based Clear Floor Polishes, D1290-95(2021) Standard Test Method for  Sediment in Water-Emulsion Polishes by Centrifuge, D1791-93(2022) Standard Test Method for  Accelerated Aging of Liquid Water-Emulsion Floor Polishes, D2834-95(2022) Standard Test Method for  Nonvolatile Matter (Total Solids) in Water-Emulsion Floor Polishes,  Solvent-Based Floor Polishes, and Polymer-Emulsion Floor Polishes, D930-89(1996)e1 Standard Test Method of Total Immersion Corrosion Test of Water-Soluble Aluminum Cleaners (Withdrawn 2001), D6215-98a Standard Guide for Removal of Oily Soils from Metal Surfaces (Withdrawn 2004), D4009-92(2017) Standard Guide for  Foam Stability of Hand Dishwashing Detergents, D5343-06(2018) Standard Guide for  Evaluating Cleaning Performance of Ceramic Tile Cleaners, D7225-13(2019)e1 Standard Guide for   Blood Cleaning Efficiency of Detergents and Washer-Disinfectors, D4488-95(2001)e1 Standard Guide for Testing Cleaning Performance of Products Intended for Use on Resilient Flooring and Washable Walls (Withdrawn 2009), D3565-89(2001) Standard Test Method for Tableware Pattern Removal by Mechanical Dishwasher Detergents (Withdrawn 2007), D1280-14 Standard Guide for  Total Immersion Corrosion Test for Soak Tank Metal Cleaners (Withdrawn 2023), D3556-23 Standard Guide for  Deposition on Glassware During Mechanical Dishwashing, D459-16 Standard Terminology Relating to  Soaps and Other Detergents, D3210-95(2016) Standard Test Method for  Comparing Colors of Films from Water-Emulsion Floor Polishes, D4330-94(2017) Standard Practice for  Evaluation of Fiberglass Boat Polish and Wax, D3207-17 Standard Test Method for  Detergent Resistance of Floor Polish Films, D1436-17 Standard Test Methods for  Application of Emulsion Floor Polishes to Substrates for Testing  Purposes, D3543-17 Standard Test Method for  Metal Glide Adhesion, D1455-17 Standard Test Method for  60 Specular Gloss of Emulsion Floor Polish, D1793-17 Standard Test Method for  Water Spotting of Emulsion Floor Polishes, D1792-17 Standard Test Method for  Long-Term Removability Properties of Emulsion Floor Polishes, D3052-17 Standard Practice for  Rating Water-Emulsion Floor Polishes, D2048-17 Standard Test Method for  Powdering of Floor Polish Films, D4002-81(2016) Standard Practice for  Evaluation of Buffable Shoe Polish, D3153-17 Standard Test Method for  Recoatability of Water-Emulsion Floor Polishes, D3206-17 Standard Test Method for  Soil Resistance of Floor Polishes, D3758-95(2017) Standard Practice for  Evaluation of Spray-Buff Products on Test Floors, D6625-13(2020) Standard Practice for  Conducting a Test of Protective Properties of Polish Applied  to a Painted Panel Using Fluorescent UV-Condensation Light- and Water-Exposure  Apparatus, D3751/D3751M-10(2017) Standard Practice for  Evaluation of Furniture Polish, D3836-13(2021) Standard Practice for Evaluation of  Automotive Polish, D4955-89(2021) Standard Practice for  Field Evaluation of Automotive Polish, D4265-21 Standard Guide for  Evaluating Stain Removal Performance in Home Laundering, D4008-19 Standard Guide for  Measuring Anti-Soil Deposition Properties of Laundry Detergents, D1172-15 Standard Guide for  pH of Aqueous Solutions of Soaps and Detergents, D1173-07(2015) Standard Test Method for Foaming Properties of Surface-Active Agents, D3050-07(2015) Standard Guide for Measuring Soil Removal from Artificially Soiled Fabrics (Not Suitable for Detergent Ranking), D2281-10(2016) Standard Test Method for  Evaluation of Wetting Agents by the Skein Test, D2024-09(2017) Standard Test Method for  Cloud Point of Nonionic Surfactants, D8179-18 Standard Guide for Characterizing Detergents for the Cleaning of Clinically-used  Medical Devices, D5237-14(2019) Standard Guide for  Evaluating Fabric Softeners, D5548-13(2020) Standard Guide for  Evaluating Color Transfer or Color Loss of Dyed Fabrics in  Laundering (Not Suitable for Detergent  or Washing Machine Rankings), D1387-89(2019) Standard Test Method for  Saponification Number (Empirical) of Synthetic and Natural  Waxes, D7389-07(2019) Standard Test Method for  Acid Number (Empirical) of Maleic Anhydride (MAH) Grafted Waxes, D3837-95(2019) Standard Practice for  Preparing a Solution of AlkaliSoluble Resins, D1342-92(2002) Standard Test Method for Paraffin-Type Hydrocarbons in Carnauba Wax (Withdrawn 2007), D3642-13(2021) Standard Test Method for  Softening Point of Certain Alkali-Soluble Resins, D1986-14(2021) Standard Test Method for  Determining the Apparent Viscosity of Polyethylene Wax, D1386-15(2022) Standard Test Method for  Acid Number (Empirical) of Synthetic and Natural Waxes, D3643-15(2022) Standard Test Method for  Acid Number of Certain Alkali-Soluble Resins, D3644-15(2022) Standard Test Method for  Acid Number of Styrene-Maleic Anhydride Resins, D3954-15(2022) Standard Test Method for  Dropping Point of Waxes, D4283-98(2022) Standard Test Method for  Viscosity of Silicone Fluids, D4103-17 Standard Practice for  Preparation of Substrate Surfaces for Coefficient of Friction  Testing, D2047-17 Standard Test Method for  Static Coefficient of Friction of Polish-Coated Flooring Surfaces  as Measured by the James Machine, D6205-20 Standard Practice for  Calibration of the James Static Coefficient of Friction Machine, D4386-95(2016) Standard Practice for  Application of Floor Polishes to Maintain Multilayer Composite  Tile or Flooring, D3757-16 Standard Guide for  Preparing Specifications for Solvent-based Floor Polishes, D3440-13(2019) Standard Guide for  Preparing Specifications for Water-Emulsion Floor Polishes, D4078-02(2021) Standard Specification for  Water Emulsion Floor Polish, D3564-22 Standard Practice for  Application of Floor Polishes to Maintain Vinyl Composition  Tile or Flooring, D2825-21e1 Standard Terminology Relating to  Polishes and Related Materials.